Synthesis and interaction of thiazolo [2, 3-a] isoquinoline analog with DNA

Abstract

A reaction between 1-(furan-2-yl)-3,4-dihydroisoquinoline and thioglycolic acid using DCC at 0⁰-5⁰C will give 10b-(furan-2-yl)-5,6-dihydro-2H-thiazolo[2,3-a]isoquinolin-3(10bH)-one [FUIQTGA]. The interaction between FUIQTGA and DNA was studied using UV Visible spectroscopy, fluorescence and Circular Dichroism (CD) techniques. Static quenching mechanism was observed from fluorescence measurements of interaction between FUIQTGA and DNA.  Circular Dichroism reveals the minor groove binding mode between FUIQTGA and DNA.