Cell line toxicity study and pharmacological screening of effective nootropic herbal formulation in rat

Abstract

The polyphyto drugs FM27; compose of Mentha piperita (10%), Ribes nigrum (20%), Cinnamomum zeylanicum (20%), Aloe vera (10%), Zingiber officinalis (5%), Glycine max (5%) and Avena sativa (30%) on memory acquisition and retention was studied using EPM, MWM and PCA test in rat using two different routes, one is per oral (FM27^O) and another one is transcranial (FM27^T). The test drug studied for in vitro safety profile screening by MTT assay in a different cell line. As per the result, cell line toxicity confirmed that formulation FM27 was non toxic in SH-SY-5Y human neuroblastoma cell line, Vero monkey normal kidney epithelial cells, and Chang liver cell line. Test drug FM27^O studied at a dose of (PO 50 and 100 mg/kg), showed graded dose response and it was observed significantly (p <0.01) effect of memory improvement comparing standard group containing Bacopa monnieri (PO 50 and 100 mg/kg), whereas transcranially applied FM27^T (10 mg/kg) showed a significant (p <0.001) effect of memory improvement comparing control group and observed similar effect like standard drug. Herbal formulations FM27 was found to be potent for treating dementia to improve memory. The cell line toxicity study report confirmed it was safe in higher dose also.